Application
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 57k peripherin protein.| Product Name | Peripherin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Perf, Prph1, |
| Product description | Peripherin is a ~57 kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name. Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. In contrast to the neurofilaments, peripherin is strongly up-regulated after nerve injury. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.1) Terao E, Janssens S, van den Bosch de Aquilar P, Portier M, Klosen P (2000) In vivo expression of the intermediate filament peripherin in rat motoneurons: modulation by inhibitory and stimulatory signals. Neuroscience 101(3):679-88. |
| Immunogen | Recombinant rat peripherin expressed in and purified from E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Mammalian species |
| Concentration | 1mg/ml |
| Gene Symbol | Prph |
|---|---|
| Alternative Names | Perf Prph1 |
| Molecular Weight(MW) | 57 |
| Tissue Specificity | Specific for the ~57kDa peripherin protein. This antibody performs well on aldehyde fixed tissues. |
Application
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 57k peripherin protein.| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Peripherin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
