Application
Immunohistochemistry of PHOX2A in human brain tissue with PHOX2A antibody at 2.5 μg/mL.Application
Immunofluorescence of PHOX2A in human brain tissue with PHOX2A antibody at 20 μg/mL.Application
Western blot analysis of PHOX2A in rat brain tissue lysate with PHOX2A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | PHOX2A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHOX2A Antibody: ARIX, FEOM2, NCAM2, PMX2A, CFEOM2, ARIX, Paired mesoderm homeobox protein 2A, ARIX1 homeodomain protein |
| Product description | PHOX2A Antibody: PHOX2A and PHOX2B (Paired mesoderm homeobox protein) are closely related, paired-homeodomain transcription factors that function as determinants of the noradrenergic phenotype during embryogenesis. PHOX2 proteins are crucial for the regulation of endogenous hydroxylases in neural crest cells and promote sympathetic neuron generation. Human PHOX2B contains one DNA binding homeobox domain and is required for the differentiation of all central and nonperipheral noradrenergic centers in the brain. In contrast, PHOX2A controls only the differentiation of the main noradrenergic center of the brain. Regulation of PHOX2 may have therapeutic utility in aging or disorders involving degeneration of noradrenergic neurons.1) Stanke M, Junghans D, Geissen M, et al. The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons. Development 1999; 126:4087-94. |
| Immunogen | PHOX2A antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human PHOX2A. The immunogen is located within the last 50 amino acids of PHOX2A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PHOX2A antibody can be used for detection of PHOX2A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHOX2A |
|---|---|
| Alternative Names | PHOX2A Antibody: ARIX FEOM2 NCAM2 PMX2A CFEOM2 ARIX Paired mesoderm homeobox protein 2A ARIX1 homeodomain protein |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | PHOX2A antibody is predicted to not cross-react with other paired homeobox family members. |
Application
Immunohistochemistry of PHOX2A in human brain tissue with PHOX2A antibody at 2.5 μg/mL.
Application
Immunofluorescence of PHOX2A in human brain tissue with PHOX2A antibody at 20 μg/mL.
Application
Western blot analysis of PHOX2A in rat brain tissue lysate with PHOX2A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | PHOX2A antibody can be used for detection of PHOX2A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PHOX2A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PHOX2A Antibody is supplied in PBS containing 0.02% sodium azide. |
