Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-PIK3CA Antibody Pab in K562 cell line lysates (35ug/lane).Application
Western blot analysis of anti-PI3KCA Antibody Pab in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane)| Product Name | PI3KCA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, PtdIns-3-kinase |
| Product description | Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.1) Tiwari, S. Nat Immunol. August; 10(8): 907?17 (2009). |
| Immunogen | This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 504-533 amino acids from the Central region of human PI3KCA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PIK3CA |
|---|---|
| Alternative Names | Phosphatidylinositol 4 5-bisphosphate 3-kinase catalytic subunit alpha isoform PI3-kinase subunit alpha PI3K-alpha PI3Kalpha PtdIns-3-kinase subunit alpha Phosphatidylinositol 4 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha PtdIns-3-kinase |
| Molecular Weight(MW) | 124 kDa |
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-PIK3CA Antibody Pab in K562 cell line lysates (35ug/lane).
Application
Western blot analysis of anti-PI3KCA Antibody Pab in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM288036