Application
Flow cytometric analysis of HepG2 cells using PK3CA Antibody (Y317)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of anti-PK3CA Antibody (Y317) in mouse heart tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PK3CA Antibody (Y317), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | PK3CA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, PtdIns-3-kinase |
| Product description | Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. This protein represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2.1) Zhao,L., Oncogene 27 (41), 5486-5496 (2008) |
| Immunogen | This PK3CA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-321 amino acids from human PK3CA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIK3CA |
|---|---|
| Alternative Names | Phosphatidylinositol 4 5-bisphosphate 3-kinase catalytic subunit alpha isoform PI3-kinase subunit alpha PI3K-alpha PI3Kalpha PtdIns-3-kinase subunit alpha Phosphatidylinositol 4 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha PtdIns-3-kinase |
| Molecular Weight(MW) | 124 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of HepG2 cells using PK3CA Antibody (Y317)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of anti-PK3CA Antibody (Y317) in mouse heart tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PK3CA Antibody (Y317), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM288180