Application
Immunohistochemistry of PIAS2 in rat brain tissue with PIAS2 antibody at 5 μg/mL.Application
Immunofluorescence of PIAS2 in rat brain tissue with PIAS2 antibody at 20 μg/mL.Application
Western blot analysis of PIAS2 in rat brain tissue lysate with PIAS2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | PIAS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIAS2 Antibody: DIP, MIZ, MIZ1, SIZ2, ARIP3, PIASX, ZMIZ4, PIASX-BETA, PIASX-ALPHA, E3 SUMO-protein ligase PIAS2, Androgen receptor-interacting protein 3 |
| Product description | PIAS2 Antibody: The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS2. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS2 binds specifically to STAT4 following IL-12 stimulation and inhibits STAT4-mediated gene activation in human T cells. PIAS2 is a potent transcriptional activator of Bcl-2, but together with Bcl-6 can suppress the expression of Bcl-2.1) Shuai K and Liu B. Regulation of gene-activation pathways by PIAS proteins in the immune system. Nat. Rev. Immunol.2005; 5:593-605. |
| Immunogen | PIAS2 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human PIAS2. The immunogen is located within amino acids 110 - 160 of PIAS2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PIAS2 antibody can be used for detection of PIAS2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIAS2 |
|---|---|
| Alternative Names | PIAS2 Antibody: DIP MIZ MIZ1 SIZ2 ARIP3 PIASX ZMIZ4 PIASX-BETA PIASX-ALPHA E3 SUMO-protein ligase PIAS2 Androgen receptor-interacting protein 3 |
Application
Immunohistochemistry of PIAS2 in rat brain tissue with PIAS2 antibody at 5 μg/mL.
Application
Immunofluorescence of PIAS2 in rat brain tissue with PIAS2 antibody at 20 μg/mL.
Application
Western blot analysis of PIAS2 in rat brain tissue lysate with PIAS2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | PIAS2 antibody can be used for detection of PIAS2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PIAS2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PIAS2 Antibody is supplied in PBS containing 0.02% sodium azide. |
