Application
Western Blot at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH19| Product Name | PIGC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylinositol N-acetylglucosaminyltransferase subunit C, 2.4.1.198, Phosphatidylinositol-glycan biosynthesis class C protein, PIG-C, PIGC, GPI2 |
| Product description | Part of the complex catalyzing the transfer of N- acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.1) Inoue N.,et al.Biochem. Biophys. Res. Commun. 226:193-199(1996). |
| Immunogen | This PIGC antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 264-294 amino acids from human PIGC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIGC |
|---|---|
| Alternative Names | Phosphatidylinositol N-acetylglucosaminyltransferase subunit C 2.4.1.198 Phosphatidylinositol-glycan biosynthesis class C protein PIG-C PIGC GPI2 |
| Molecular Weight(MW) | 34 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH19| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
