Application
Immunohistochemistry of PIKE in mouse brain tissue with PIKE antibody at 10 μg/mL.Application
Western blot analysis of PIKE in mouse brain cell lysates with PIKE antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | PIKE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIKE Antibody: PIKE, GGAP2, CENTG1, KIAA0167, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, Centaurin-gamma-1, AGAP-2 |
| Product description | PIKE Antibody: Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.1) Ye K, Hurt KJ, Wu FY, et al. Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N. Cell 2000; 103:919-30. |
| Immunogen | PIKE antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human PIKE. The immunogen is located within the last 50 amino acids of PIKE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| PIKE antibody can be used for detection of PIKE by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AGAP2 |
|---|---|
| Alternative Names | PIKE Antibody: PIKE GGAP2 CENTG1 KIAA0167 Arf-GAP with GTPase ANK repeat and PH domain-containing protein 2 Centaurin-gamma-1 AGAP-2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (86%) |
| Tissue Specificity | Anti-PIKE will detect both PIKE-L and PIKE-A isoforms. |
Application
Immunohistochemistry of PIKE in mouse brain tissue with PIKE antibody at 10 μg/mL.
Application
Western blot analysis of PIKE in mouse brain cell lysates with PIKE antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | PIKE antibody can be used for detection of PIKE by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PIKE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PIKE Antibody is supplied in PBS containing 0.02% sodium azide. |
