Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human HepG2 at 1 ug/ml.| Product Name | PKLR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKLR, PK1, PKL, RPK, PKR, PKRL |
| Product description | PKLR is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Alternatively spliced transcript variants encoding distinct isoforms have been described.1) Pendergrass, D.C., (2006) IUBMB Life 58 (1), 31-38. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PKLR. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| PKLR antibody can be used for detection of PKLR by ELISA at 1:62500. PKLR antibody can be used for detection of PKLR by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | C.elegansDogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PKLR |
|---|---|
| Alternative Names | PKLR PK1 PKL RPK PKR PKRL |
| Molecular Weight(MW) | 58 kDa, 62 kDa |
Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PKLR antibody can be used for detection of PKLR by ELISA at 1:62500. PKLR antibody can be used for detection of PKLR by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PKLR antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
