Application
Antibody is used in Western blot to detect PKLR in mouse liver tissue lysate.Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PKLR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pyruvate kinase PKLR, Pyruvate kinase 1, Pyruvate kinase isozymes L/R, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase, PKLR, PK1, PKL |
| Product description | PKLR is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).1) Wang, H., et al., Diabetes 51(9):2861-2865 (2002). |
| Immunogen | This PKLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-551 amino acids from the C-terminal region of human PKLR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PKLR |
|---|---|
| Alternative Names | Pyruvate kinase PKLR Pyruvate kinase 1 Pyruvate kinase isozymes L/R R-type/L-type pyruvate kinase Red cell/liver pyruvate kinase PKLR PK1 PKL |
| Molecular Weight(MW) | 62 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Antibody is used in Western blot to detect PKLR in mouse liver tissue lysate.
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
