Application
Antibody used in WB on Human HepG2 at 5 ug/ml.| Product Name | POLR2B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR2B, RPB2, POL2RB, hsRPB2, hRPB140 |
| Product description | POLR2B is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.1) Langelier, M.F., et al., (2005) (er) Nucleic Acids Res. 33 (8), 2629-2639. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR2B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| POLR2B antibody can be used for detection of POLR2B by ELISA at 1:312500. POLR2B antibody can be used for detection of POLR2B by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | POLR2B |
|---|---|
| Alternative Names | POLR2B RPB2 POL2RB hsRPB2 hRPB140 |
| Molecular Weight(MW) | 134 kDa |
Application
Antibody used in WB on Human HepG2 at 5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | POLR2B antibody can be used for detection of POLR2B by ELISA at 1:312500. POLR2B antibody can be used for detection of POLR2B by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store POLR2B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
