WB
Western blot analysis of various lysates using POLR2B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffinembedded Rat liver using POLR2B Rabbit pAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse liver using POLR2B RabbitpAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.| Product Name | POLR2B Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POLR2B |
|---|---|
| Gene Synonyms | RPB2 POL2RB hRPB140 |
| Gene Full Name | RNA polymerase II subunit B |
| Gene Summary | This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | RPB2 POL2RB hRPB140 POLR2B |
| Molecular Weight(MW) | 134kDa(Observed band size:134kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of various lysates using POLR2B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffinembedded Rat liver using POLR2B Rabbit pAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse liver using POLR2B RabbitpAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
