WB
A431 cells were subjected to SDS PAGE followed by western blot with POLR2B-Specific antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human brain using POLR2B-Specific antibody at dilution of 1:400 (under 10x lens).IP
IP result of anti-POLR2B-Specific (IP:4ug; Detection:1:300) with HeLa cells lysate 3000ug.| Product Name | POLR2B-Specific Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:2000 IHC:1:20-1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POLR2B |
|---|---|
| Gene Synonyms | RPB2 POL2RB hRPB140 |
| Gene Full Name | RNA polymerase II subunit B |
| Gene Summary | This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | hRPB140 hsRPB2 POL2RB POLR2B POLR2B-Specific RNA polymerase II subunit 2 RNA polymerase II subunit B2 RPB2 |
| Molecular Weight(MW) | 140 kDa |
| Cellular Localization | DNA-directed RNA polymerase,Nucleus. |
WB
A431 cells were subjected to SDS PAGE followed by western blot with POLR2B-Specific antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human brain using POLR2B-Specific antibody at dilution of 1:400 (under 10x lens).
IP
IP result of anti-POLR2B-Specific (IP:4ug; Detection:1:300) with HeLa cells lysate 3000ug.| Application Notes | WB:1:500-1:2000 IHC:1:20-1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
