Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.Application
Antibody used in IHC on Human kidney.| Product Name | POU4F3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | POU4F3 is capable of activating both BDNF and NT-3 promoters in inner ear sensory epithelial cell lines. Mutant POU4F3 loses most of its transcriptional activity and most of its ability to bind to DNA. The mutation causes autosomal-dominant nonsyndromic hearing loss and eventually leads to hair cell morbidity in affected family members.1) Weiss, S., et al., (2003) Mol. Cell. Biol. 23 (22), 7957-7964. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU4F3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| POU4F3 antibody can be used for detection of POU4F3 by ELISA at 1:312500. POU4F3 antibody can be used for detection of POU4F3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | POU4F3 |
|---|---|
| Alternative Names | POU4F3 BRN3C DFNA15 |
| Molecular Weight(MW) | 37 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | POU4F3 antibody can be used for detection of POU4F3 by ELISA at 1:312500. POU4F3 antibody can be used for detection of POU4F3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store POU4F3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
