Application
Immunohistochemistry of POU5F1 in rat liver tissue with POU5F1 antibody at 5 μgg/mL.Application
Western blot analysis of POU5F1 in mouse liver tissue lysate with POU5F1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | POU5F1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | POU5F1 Antibody: POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.1) Scholer HR, Ruppert S, Suzuki N, et al. New type of POU domain in germ line-specific protein Oct-4. Nature1990; 344:435-9. |
| Immunogen | POU5F1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human POU5F1. The immunogen is located within amino acids 260 - 310 of POU5F1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| POU5F1 antibody can be used for detection of POU5F1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POU5F1 |
|---|---|
| Alternative Names | POU5F1 Antibody: OCT3 OCT4 OTF3 OTF4 OTF-3 Oct-3 Oct-4 OCT3 POU domain class 5 transcription factor 1 Octamer-binding protein 3 41915 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%) |
Application
Immunohistochemistry of POU5F1 in rat liver tissue with POU5F1 antibody at 5 μgg/mL.
Application
Western blot analysis of POU5F1 in mouse liver tissue lysate with POU5F1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | POU5F1 antibody can be used for detection of POU5F1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | POU5F1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | POU5F1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM288551