Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Product Name | POU5F1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm1) Suo, G., et al., (2005) Biochem. Biophys. Res. Commun. 337 (4), 1047-1051. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU5F1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| POU5F1 antibody can be used for detection of POU5F1 by ELISA at 1:62500. POU5F1 antibody can be used for detection of POU5F1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | POU5F1 |
|---|---|
| Alternative Names | POU5F1 OCT3 OCT4 OTF3 OTF4 OTF-3 Oct-3 Oct-4 |
| Molecular Weight(MW) | 39 kDa |
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | POU5F1 antibody can be used for detection of POU5F1 by ELISA at 1:62500. POU5F1 antibody can be used for detection of POU5F1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store POU5F1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
Data sheet for OM288552