Application
Western blot analysis of PRTFDC1 in human brain tissue lysate with PRTFDC1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.| Product Name | PRTFDC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRTFDC1 Antibody: HHGP, HHGP, Phosphoribosyltransferase domain-containing protein 1 |
| Product description | PRTFDC1 Antibody: Phosphoribosyl transferase domain containing 1 (PRTFDC1) is highly homologous to the hypoxanthine phosphoribosyltransferase (HPRT1) and may have arisen from a gene duplication event of a common ancestor gene. Recently, it was shown that CpG islands in the PRTFDC1 promoter could be hypermethylated in ovarian cancers and oral squamous-cell carcinomas (OSCC), leading to gene silencing. Restoration of PRTFDC1 expression in OSCC inhibited cell growth in colony-formation assays, while knockdown of PRTFDC1 expression in OSCC that expressed the gene promoted cell growth. These results suggest that PRTFDC1 can act as a tumor-suppressor gene. At least three isoforms of PRTFDC1 are known to exist.1) Nicklas JA. Pseudogenes of the human HPRT1 gene. Environ. Mol. Mutagen2006; 47:212-8. |
| Immunogen | PRTFDC1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human PRTFDC1. The immunogen is located within amino acids 90 - 140 of PRTFDC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PRTFDC1 antibody can be used for detection of PRTFDC1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRTFDC1 |
|---|---|
| Alternative Names | PRTFDC1 Antibody: HHGP HHGP Phosphoribosyltransferase domain-containing protein 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (86%), Pig: (86%), Bovine: (86%), Mouse: (86%) |
Application
Western blot analysis of PRTFDC1 in human brain tissue lysate with PRTFDC1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | PRTFDC1 antibody can be used for detection of PRTFDC1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PRTFDC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PRTFDC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
