PRTFDC1 belongs to the purine/pyrimidine phosphoribosyltransferase family. Epigenetic silencing of PRTFDC1 by hypermethylation of the CpG islands leads to a loss of PRTFDC1 function, which might be involved in squamous cell oral carcinogenesis.1) Suzuki, E., (2007) Oncogene 26 (57), 7921-7932.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRTFDC1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PRTFDC1 antibody can be used for detection of PRTFDC1 by ELISA at 1:1562500. PRTFDC1 antibody can be used for detection of PRTFDC1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
PRTFDC1 antibody can be used for detection of PRTFDC1 by ELISA at 1:1562500. PRTFDC1 antibody can be used for detection of PRTFDC1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PRTFDC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.