Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | PTK2B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTK2B, PKB, PTK, CAKB, FAK2, PYK2, CADTK, FADK2, RAFTK |
| Product description | PTK2B encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.1) Ling, S., et al., (2004) Am. J. Physiol., Cell Physiol. 287 (3), C698-C706. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PTK2B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PTK2B antibody can be used for detection of PTK2B by ELISA at 1:312500. PTK2B antibody can be used for detection of PTK2B by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTK2B |
|---|---|
| Alternative Names | PTK2B PKB PTK CAKB FAK2 PYK2 CADTK FADK2 RAFTK |
| Molecular Weight(MW) | 116 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PTK2B antibody can be used for detection of PTK2B by ELISA at 1:312500. PTK2B antibody can be used for detection of PTK2B by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PTK2B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
