Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 3T3 cells (Lane 2) and HepG2 cells (Lane 3), using PYK2 (Phospho-Tyr579) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | PYK2 (phospho Tyr579) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.1) Lev S., Nature 376:737-745(1995). |
| Immunogen | PYK2 (phospho Tyr579) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 579 (E-D-Y (p)-Y-K) derived from Human PYK2. |
| Modification | p-Tyr579 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTK2B |
|---|---|
| Alternative Names | CADTK FADK 2 FAK2 PTK2B RAFTK |
| Molecular Weight(MW) | 116 kDa |
| Tissue Specificity | The antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 579. |
| Entrez Gene | 2185 |
|---|---|
| Protein Accession | NP_004094.3 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 3T3 cells (Lane 2) and HepG2 cells (Lane 3), using PYK2 (Phospho-Tyr579) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
