Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with Serum using Pyk2 (Phospho-Tyr402).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Pyk2 (Phospho-Tyr402). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Pyk2 (Phospho-Tyr402).| Product Name | Pyk2 (phospho Tyr402) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.1) Gluck SL, et al. (2004) J Clin Invest; 114 (12): 1696-1699Benzing T, et al. (2001) Proc Natl Acad Sci U S A; 98 (17): 9784-9789Tian D, et al. (2002) Mol Cell Biol; 22 (8): 2650-2662Lu Z, et al. (2001) Mol Cell Biol; 21 (12): 4016-4031Krishnan HH, et al. (2006) J Virol; 80 (3): 1167-1180 |
| Immunogen | Pyk2 (Phospho-Tyr402) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 402 (D-I-Y (p) -A-E) derived from Human Pyk2. |
| Modification | p-Tyr402 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTK2B |
|---|---|
| Alternative Names | PKB PTK CAKB FAK2 PYK2 CADTK FADK2 RAFTK MAPTL MTBT1 TAU FADK 2 Focal adhesion kinase 2 PTK2B Proline-rich tyrosine kinase 2 RAFTK |
| Molecular Weight(MW) | 116 kDa |
| Tissue Specificity | This antibody detects endogenous level of Pyk2 only when phosphorylated at tyrosine 402. |
| Entrez Gene | 2185 |
|---|---|
| Protein Accession | NP_004094.3 |
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with Serum using Pyk2 (Phospho-Tyr402).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Pyk2 (Phospho-Tyr402). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Pyk2 (Phospho-Tyr402).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
