Application
Immunohistochemistry of PTK7 in human colon tissue with PTK7 antibody at 2.5 μg/mL.Application
Western blot analysis of PTK7 in (A) human colon, (B) mouse kidney and (C) rat liver tissue lysate with PTK7 antibody at 1 μg/mL.Application
Immunofluorescence of PTK7 in Human Colon cells with PTK7 antibody at 20 μg/mL.| Product Name | PTK7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTK7 Antibody: CCK4, CCK-4, CCK4, Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4 |
| Product description | PTK7 Antibody: Protein-tyrosine kinases (PTKs) play important roles in regulating cell proliferation and differentiation during development. One member of the PTK family, PTK7, has been suggested to regulate the planar cell polarity (PCP) pathway in vertebrates and may play a role in neural convergent extension and neural tube closure. PTK7 has also been implicated in the development of cancer. Loss of PTK7 expression was seen in several melanoma cell lines and biopsies. Conversely, high-throughput analysis of acute myeloid leukemia samples showed an increased level of PTK7 expression compared to normal bone marrow and purified CD34+ cells. Multiple isoforms of PTK7 are known to exist.1) Park SK, Lee HS and Lee ST. Characterization of the human full-length PTK7 cDNA encoding a receptor protein kinase-like molecule closely related to chick KLG. J. Biochem.1996; 119:235-9. |
| Immunogen | PTK7 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human PTK7. The immunogen is located within amino acids 880 - 930 of PTK7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PTK7 antibody can be used for detection of PTK7 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTK7 |
|---|---|
| Alternative Names | PTK7 Antibody: CCK4 CCK-4 CCK4 Inactive tyrosine-protein kinase 7 Colon carcinoma kinase 4 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (84%) |
Application
Immunohistochemistry of PTK7 in human colon tissue with PTK7 antibody at 2.5 μg/mL.
Application
Western blot analysis of PTK7 in (A) human colon, (B) mouse kidney and (C) rat liver tissue lysate with PTK7 antibody at 1 μg/mL.
Application
Immunofluorescence of PTK7 in Human Colon cells with PTK7 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | PTK7 antibody can be used for detection of PTK7 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PTK7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PTK7 Antibody is supplied in PBS containing 0.02% sodium azide. |
