Application
Immunohistochemical analysis of paraffin - embedded human bladder carcinoma (top), human breast carcinoma (middle), and human skin carcinoma (bottom) showing cytoplasmic localization using Rab25 antibody with DAB staining.Application
Western blot analysis using Rab25 monoclonal antibody against truncated Rab25 recombinant protein human overy carcinoma stomach carcinoma breast carcinoma tissue lysate.| Product Name | Rab25 Antibody [3F12F3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.1) JR Goldenring, KR Shen, HD Vaughan. et al. J. Biol. Chem,1993,268(25):18419-18422 |
| Immunogen | Ni-NTA purified recombinant Rab25 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAB25 |
|---|---|
| Alternative Names | CATX8 CATX-8CATX-8 RAB11C |
Application
Immunohistochemical analysis of paraffin - embedded human bladder carcinoma (top), human breast carcinoma (middle), and human skin carcinoma (bottom) showing cytoplasmic localization using Rab25 antibody with DAB staining.
Application
Western blot analysis using Rab25 monoclonal antibody against truncated Rab25 recombinant protein human overy carcinoma stomach carcinoma breast carcinoma tissue lysate.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Rab25 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide. |
