Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.Application
Antibody used in WB on Hela, HEK293T, Xenopus laevis egg extract, mouse embryonic at 1:500 (Lane1: 25ug Hela lysate, Lane2: 25ug HEK293T lysate, Lane3: 25ug Xenopus laevis egg extract, Lane4: 25ug mouse embryonic stem cells lysate).| Product Name | RAD17 Antibody |
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| Antibody Type | Primary Antibodies |
| Product description | RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.1) Tsao, C.C., (2004) EMBO J. 23 (23), 4660-4669. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RAD17. |
| Clonality | Polyclonal |
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| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RAD17 antibody can be used for detection of RAD17 by ELISA at 1:62500. RAD17 antibody can be used for detection of RAD17 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAD17 |
|---|---|
| Alternative Names | RAD17 CCYC HRAD17 R24L RAD17Sp Rad24 RAD24 RAD17SP |
| Molecular Weight(MW) | 66 kDa, 76 kDa, 76 kDa, 77 kDa, 57 kDa, 76 kDa, 76 kDa, 76 kDa |
Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.
Application
Antibody used in WB on Hela, HEK293T, Xenopus laevis egg extract, mouse embryonic at 1:500 (Lane1: 25ug Hela lysate, Lane2: 25ug HEK293T lysate, Lane3: 25ug Xenopus laevis egg extract, Lane4: 25ug mouse embryonic stem cells lysate).| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
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| Application Notes | RAD17 antibody can be used for detection of RAD17 by ELISA at 1:62500. RAD17 antibody can be used for detection of RAD17 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RAD17 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
