Application
Western blot analysis in mouse lung tissue lysates (35ug/lane).Application
Western Blot at 1:2000 dilution + KG-1 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | RASSF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.1) El-Kalla, M., et al. Oncogene 29(42):5729-5740(2010) |
| Immunogen | This RASSF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human RASSF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RASSF1 |
|---|---|
| Alternative Names | Ras association domain-containing protein 1 RASSF1 {ECO:0000312|EMBL:AAF351282} |
| Molecular Weight(MW) | 39 kDa |
Application
Western blot analysis in mouse lung tissue lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution + KG-1 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
