Application
Antibody is used in Western blot to detect RCAN1/DSCR1 in endothelial tissue lysate.Application
Endothelial cell lysate transferred to membrane was incubated with primary antibody at a 1:500 dilution in 2% BSA in TBST at 4deg C overnight. Data courtesy of Dr. Katherine Healey, NWCRF Institute, School of Biological Sciences, University of Wales Bangor.| Product Name | RCAN1/DSCR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Calcipressin-1, Adapt78, Down syndrome critical region protein 1, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Regulator of calcineurin 1, RCAN1, ADAPT78, CSP1, DSC1, DSCR1 |
| Product description | DSCR1 interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. The gene for this protein is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of DSCR1 may lead to neurofibrillary tangles such as those associated with Alzheimer disease.1) Minami, T., et al., J. Biol. Chem. 279(48):50537-50554 (2004). Yao, Y.G., et al., Biochem. Biophys. Res. Commun. 321(3):648-656 (2004). Michtalik, H.J., et al., Free Radic. Biol. Med. 37(4):454-462 (2004). Hesser, B.A., et al., Blood 104(1):149-158 (2004). Iizuka, M., et al., Biochem. Biophys. Res. Commun. 41(4):334-344 (2004). |
| Immunogen | This RCAN1/DSCR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-252 amino acids from the C-terminal region of human DSCR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | RCAN1 |
|---|---|
| Alternative Names | Calcipressin-1 Adapt78 Down syndrome critical region protein 1 Myocyte-enriched calcineurin-interacting protein 1 MCIP1 Regulator of calcineurin 1 RCAN1 ADAPT78 CSP1 DSC1 DSCR1 |
| Molecular Weight(MW) | 28 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Antibody is used in Western blot to detect RCAN1/DSCR1 in endothelial tissue lysate.
Application
Endothelial cell lysate transferred to membrane was incubated with primary antibody at a 1:500 dilution in 2% BSA in TBST at 4deg C overnight. Data courtesy of Dr. Katherine Healey, NWCRF Institute, School of Biological Sciences, University of Wales Bangor.| Application Notes | For WB starting dilution is: 1:1000: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
