Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Product Name | REC8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | REC8, HR21spB, MGC950, REC8L1, Rec8p |
| Product description | REC8 is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.1) Xu, H., (2005) Dev. Cell 8 (6), 949-961. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human REC8. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| REC8 antibody can be used for detection of REC8 by ELISA at 1:1562500. REC8 antibody can be used for detection of REC8 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | REC8 |
|---|---|
| Alternative Names | REC8 HR21spB MGC950 REC8L1 Rec8p |
| Molecular Weight(MW) | 62 kDa |
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | REC8 antibody can be used for detection of REC8 by ELISA at 1:1562500. REC8 antibody can be used for detection of REC8 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store REC8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
