Application
Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | RHOC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rho-related GTP-binding protein RhoC, Rho cDNA clone 9, h9, RHOC, ARH9, ARHC |
| Product description | This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.1) Jiang, L., et al. Int. J. Cancer 127(3):505-512(2010) |
| Immunogen | This RHOC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the C-terminal region of human RHOC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RHOC |
|---|---|
| Alternative Names | Rho-related GTP-binding protein RhoC Rho cDNA clone 9 h9 RHOC ARH9 ARHC |
| Molecular Weight(MW) | 22 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
