Application
Immunofluorescence of RICK in K562 cells with RICK antibody at 20 μg/mL.Application
Immunocytochemistry of RICK in K562 cells with RICK antibody at 5 μg/mL.Application
Western blot analysis of RICK in (A) 3T3 and (B) K562 cell lysate with RICK antibody at 0.5 μg/mL| Product Name | RICK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RICK Antibody: CCK, RICK, RIP2, CARD3, GIG30, CARDIAK, UNQ277/PRO314/PRO34092, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase |
| Product description | RICK Antibody: Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-κB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1β. The messenger RNA of RICK is expressed in multiple human tissues.1) Inohara N, del Peso L, Koseki T, Chen S, Nunez G. RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J Biol Chem 1998;273:12296-300 |
| Immunogen | RICK antibody was raised against a 15 amino acid peptide near the carboxy terminus of human RICK. The immunogen is located within the last 50 amino acids of RICK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| RICK antibody can be used for detection of RICK by Western blot at 0.5 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RIPK2 |
|---|---|
| Alternative Names | RICK Antibody: CCK RICK RIP2 CARD3 GIG30 CARDIAK UNQ277/PRO314/PRO34092 CARD-containing interleukin-1 beta-converting enzyme-associated kinase CARD-containing IL-1 beta ICE-kinase |
| Molecular Weight(MW) | Predicted: 60 kDa Observed: 58 kDa |
| Sequence Similarities | 3) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunofluorescence of RICK in K562 cells with RICK antibody at 20 μg/mL.
Application
Immunocytochemistry of RICK in K562 cells with RICK antibody at 5 μg/mL.
Application
Western blot analysis of RICK in (A) 3T3 and (B) K562 cell lysate with RICK antibody at 0.5 μg/mL| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | RICK antibody can be used for detection of RICK by Western blot at 0.5 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RICK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RICK Antibody is supplied in PBS containing 0.02% sodium azide. |
