Application
Immunohistochemistry of RIP3 in rat kidney tissue with RIP3 antibody at 5 μg/mL.Application
Immunofluorescence of RIP3 in Rat Kidney cells with RIP3 antibody at 20 μg/mL.Application
Western blot analysis of RIP3 in HeLa cell lysate with RIP3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | RIP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RIP3 Antibody: Rip3, AW107945, 2610528K09Rik, Rip3, RIP-like protein kinase 3, RIP-3 |
| Product description | RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.1) Yu PW, Huang BC, Shen M, Quast J, Chan E, Xu X, Nolan GP, Payan DG, Luo Y. Identification of RIP3, a RIP-like kinase that activates apoptosis and NFκB. Curr Biol. 1999;9(10):539-42. |
| Immunogen | RIP3 antibody was raised against a 14 amino acid peptide near the carboxy terminus of murine RIP3. The immunogen is located within the last 50 amino acids of RIP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:1000 IF:1:100~1:500 IHC-p:1:50~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Ripk3 |
|---|---|
| Alternative Names | RIP3 Antibody: Rip3 AW107945 2610528K09Rik Rip3 RIP-like protein kinase 3 RIP-3 |
| Molecular Weight(MW) | Predicted: 57 kDa Observed: 60 kDa |
| Sequence Similarities | 2) Cat. No. 1212 - 3T3 Cell Lysate |
Application
Immunohistochemistry of RIP3 in rat kidney tissue with RIP3 antibody at 5 μg/mL.
Application
Immunofluorescence of RIP3 in Rat Kidney cells with RIP3 antibody at 20 μg/mL.
Application
Western blot analysis of RIP3 in HeLa cell lysate with RIP3 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:1000 IF:1:100~1:500 IHC-p:1:50~1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | RIP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RIP3 Antibody is supplied in PBS containing 0.02% sodium azide. |
