Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Product Name | RMI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RMI1, BLAP75, C9orf76, FLJ12888, RP11-346I8.1, FAAP75 |
| Product description | RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. RMI1 is required for BLM phosphorylation during mitosis. Within the BLM complex, RMI1 is required for BLM and TOP3A stability.RMI1 is a component of protein complexes that limit DNA crossover formation via the dissolution of double Holliday junctions (Raynard et al., 2006 [PubMed 16595695]).1) Broberg, K., (2007) Cancer Lett. 258 (1), 38-44. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RMI1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RMI1 antibody can be used for detection of RMI1 by ELISA at 1:1562500. RMI1 antibody can be used for detection of RMI1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RMI1 |
|---|---|
| Alternative Names | RMI1 BLAP75 C9orf76 FLJ12888 RP11-346I8.1 FAAP75 |
| Molecular Weight(MW) | 70 kDa |
Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | RMI1 antibody can be used for detection of RMI1 by ELISA at 1:1562500. RMI1 antibody can be used for detection of RMI1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RMI1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
