Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.Application
Antibody used in WB on Human A549 at: 1:800 (Lane1: 50 ug hormoxia A549 lysate, Lane2: 50 ug hypoxia A549 lysate, Lane3: 50 ug hormoxia A549 lysate (+scrambled siRNA), Lane4: 50 ug hypoxia A549 lysate (+scrambled siRNA), Lane5: 50 ug hormoxia A549 lysate (RNF31 siRNA), Lane6: 50 ug hypoxia A549 lysate (RNF31 siRNA)).| Product Name | RNF31 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF31, FLJ10111, FLJ23501, MGC19975, ZIBRA, HOIP |
| Product description | PWP2 contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.1) Kirisako, T., (2006) EMBO J. 25 (20), 4877-4887. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF31. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RNF31 antibody can be used for detection of RNF31 by ELISA at 1:312500. RNF31 antibody can be used for detection of RNF31 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF31 |
|---|---|
| Alternative Names | RNF31 FLJ10111 FLJ23501 MGC19975 ZIBRA HOIP |
| Molecular Weight(MW) | 120 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Application
Antibody used in WB on Human A549 at: 1:800 (Lane1: 50 ug hormoxia A549 lysate, Lane2: 50 ug hypoxia A549 lysate, Lane3: 50 ug hormoxia A549 lysate (+scrambled siRNA), Lane4: 50 ug hypoxia A549 lysate (+scrambled siRNA), Lane5: 50 ug hormoxia A549 lysate (RNF31 siRNA), Lane6: 50 ug hypoxia A549 lysate (RNF31 siRNA)).| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | RNF31 antibody can be used for detection of RNF31 by ELISA at 1:312500. RNF31 antibody can be used for detection of RNF31 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNF31 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
