Immunoblot
Lane1: 50 ug hormoxia A549 lysateLane2: 50 ug hypoxia A549 lysateLane3: 50 ug hormoxia A549 lysate (+scrambled siRNA)Lane4: 50 ug hypoxia A549 lysate (+scrambled siRNA)Lane5: 50 ug hormoxia A549 lysate (RNF31 siRNA)Lane6: 50 ug hypoxia A549 lysate (RNF31 siRNA)Primary Antibody Dilution: 1:800Secondary Antibody: Goat anti rabbit HRP Secondary Antibody Dilution: 1:10000Gene Name: RNF31Submitted by: Markus Queisser, Northwestern UniversityWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-RNF31 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ10111, FLJ23501, MGC19975, ZIBRA, HOIP |
| Immunogen | The immunogen for anti-RNF31 antibody: synthetic peptide directed towards the middle region of human RNF31 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF31 |
|---|---|
| Gene Synonyms | FLJ10111 FLJ23501 MGC19975 ZIBRA HOIP |
| Gene Full Name | Ring finger protein 31 |
| Gene Summary | PWP2 contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. |
| Alternative Names | FLJ10111 FLJ23501 MGC19975 ZIBRA HOIP |
| Molecular Weight(MW) | 120kDa |
| Sequence | 1072 amino acids |
Immunoblot
Lane1: 50 ug hormoxia A549 lysateLane2: 50 ug hypoxia A549 lysateLane3: 50 ug hormoxia A549 lysate (+scrambled siRNA)Lane4: 50 ug hypoxia A549 lysate (+scrambled siRNA)Lane5: 50 ug hormoxia A549 lysate (RNF31 siRNA)Lane6: 50 ug hypoxia A549 lysate (RNF31 siRNA)Primary Antibody Dilution: 1:800Secondary Antibody: Goat anti rabbit HRP Secondary Antibody Dilution: 1:10000Gene Name: RNF31Submitted by: Markus Queisser, Northwestern University
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
