Application
Western blot analysis in CEM cell line lysates (35ug/lane).Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | RNF31 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase RNF31, 632-, HOIL-1-interacting protein, HOIP, RING finger protein 31, Zinc in-between-RING-finger ubiquitin-associated domain protein, RNF31, ZIBRA |
| Product description | The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.1) Silva, L.K., et al. Eur. J. Hum. Genet. (2010) In pressEhrlund, A., et al. Mol. Cell. Biol. 29(8):2230-2242(2009) Tokunaga, F., et al. Nat. Cell Biol. 11(2):123-132(2009) Kirisako, T., et al. EMBO J. 25(20):4877-4887(2006) Lim, J., et al. Cell 125(4):801-814(2006) |
| Immunogen | This RNF31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-983 amino acids from the C-terminal region of human RNF31. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF31 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase RNF31 632- HOIL-1-interacting protein HOIP RING finger protein 31 Zinc in-between-RING-finger ubiquitin-associated domain protein RNF31 ZIBRA |
| Molecular Weight(MW) | 120 kDa |
Application
Western blot analysis in CEM cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
