Application
Immunohistochemistry staining of RNF34 in brain cortex tissue using RNF34 Antibody.| Product Name | RNF34 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF34, CARP1, Caspase regulator CARP1, CARP-1, FYVE-RING finger protein Momo, HRFI, RFI, RIF, RIFF, RING finger protein 34, RING finger protein RIFF |
| Product description | RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF-κB activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RNF34 protects cells against apoptosis induced by TNF and may bind phosphatidylinositol phosphates. RNF34 is localized to both the nucleus and the cytoplasm. In the nucleus, a distinct speckled pattern is observed by immunofluorescence microscopy. In the cytoplasm, the protein is associated with membranes. RNF34 is expressed ubiquitously and can be detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Multiple transcript variants have been reported for this protein. |
| Immunogen | RNF34 antibody was raised against amino acids 1 - 373 of RNF34 (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| RNF34 antibody can be used in ELISA starting at 10 - 20 μg/mL, Western Blot starting at 1 - 2 μg/mL, and immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RNF34 |
|---|---|
| Alternative Names | RNF34 CARP1 Caspase regulator CARP1 CARP-1 FYVE-RING finger protein Momo HRFI RFI RIF RIFF RING finger protein 34 RING finger protein RIFF |
Application
Immunohistochemistry staining of RNF34 in brain cortex tissue using RNF34 Antibody.| Application Notes | RNF34 antibody can be used in ELISA starting at 10 - 20 μg/mL, Western Blot starting at 1 - 2 μg/mL, and immunohistochemistry starting at 5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store RNF34 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
