Application
Western blot analysis in Hela cell line lysates (35ug/lane).| Product Name | RNF34 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase RNF34, 632- {ECO:0000269|PubMed:25012219, ECO:0000269|Ref13}, Caspase regulator CARP1, Caspases-8 and -10-associated RING finger protein 1, CARP-1, FYVE-RING finger protein Momo, RNF34 ( HGNC:17297 ) |
| Product description | The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported.1) Erlbruch, A., et al. Proteomics 10(16):2890-2900(2010) |
| Immunogen | This RNF34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-311 amino acids from the C-terminal region of human RNF34. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF34 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase RNF34 632- {ECO:0000269|PubMed:25012219 ECO:0000269|Ref13} Caspase regulator CARP1 Caspases-8 and -10-associated RING finger protein 1 CARP-1 FYVE-RING finger protein Momo RNF34 ( HGNC:17297 ) |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in Hela cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
