Application
Western blot analysis of RNF8 in human lung tissue lysate with RNF8 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of RNF8 in rat lung tissue with RNF8 antibody at 20 μg/mL.| Product Name | RNF8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RNF8 Antibody: RNF8 was identified as a ubiquitin ligase (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A histone ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.1) Ito K, Adachi S, Iwakami R, et al. N-Terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8. Eur. J. Biochem. 2001; 268:2725-32. |
| Immunogen | RNF8 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human RNF8. The immunogen is located within amino acids 330 - 380 of RNF8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| RNF8 antibody can be used for detection of RNF8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF8 |
|---|---|
| Alternative Names | RNF8 Antibody: hRNF8 KIAA0646 E3 ubiquitin-protein ligase RNF8 RING finger protein 8 hRNF8 |
| Molecular Weight(MW) | Predicted: 46, 53 kDa Observed: 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (92%) |
| Tissue Specificity | At least three isoforms of RNF8 are known to exist; this antibody will detect all three isoforms. |
Application
Western blot analysis of RNF8 in human lung tissue lysate with RNF8 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of RNF8 in rat lung tissue with RNF8 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | RNF8 antibody can be used for detection of RNF8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | RNF8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RNF8 Antibody is supplied in PBS containing 0.02% sodium azide. |
