Application
Antibody used in WB.Application
Antibody used in IHC on Human Heart.| Product Name | RNF8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF8, FLJ12013, KIAA0646, hRNF8 |
| Product description | RNF8 contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins.The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported.1) Lehner, B. (2004) Genome Res. 14 (7), 1315-1323. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF8. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| RNF8 antibody can be used for detection of RNF8 by ELISA at 1:312500. RNF8 antibody can be used for detection of RNF8 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RNF8 |
|---|---|
| Alternative Names | RNF8 FLJ12013 KIAA0646 hRNF8 |
| Molecular Weight(MW) | 55 kDa, 51 kDa |
Application
Antibody used in WB.
Application
Antibody used in IHC on Human Heart.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | RNF8 antibody can be used for detection of RNF8 by ELISA at 1:312500. RNF8 antibody can be used for detection of RNF8 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNF8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
