Application
Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | ROBO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Roundabout homolog 1, Deleted in U twenty twenty, H-Robo-1, ROBO1, DUTT1 |
| Product description | Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.1) Kidd T.,et al.Cell 92:205-215(1998). |
| Immunogen | This ROBO1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1097-1130 amino acids from human ROBO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000-1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ROBO1 |
|---|---|
| Alternative Names | Roundabout homolog 1 Deleted in U twenty twenty H-Robo-1 ROBO1 DUTT1 |
| Molecular Weight(MW) | 181 kDa |
Application
Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000-1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM290213