WB
Y79 cells were subjected to SDS PAGE followed by western blot with ROBO1 Antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using ROBO1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ROBO1 antibody at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ROBO1 antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | ROBO1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ROBO1 protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:200-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 IF-P:1:50-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ROBO1 |
|---|---|
| Gene Synonyms | NORS NYS8 SAX3 CPHD8 DUTT1 |
| Gene Full Name | roundabout guidance receptor 1 |
| Gene Summary | Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Molecular Weight(MW) | 200-250kDa |
| Cellular Localization | Cell membrane. |
WB
Y79 cells were subjected to SDS PAGE followed by western blot with ROBO1 Antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using ROBO1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ROBO1 antibody at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ROBO1 antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:200-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 IF-P:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM643873