Application
Immunocytochemistry staining of Daudi cells using SARM antibody at 2 μg/mL.Application
Immunofluorescence of SARM in Daudi cells with SARM antibody at 2 μg/mL.Application
Western blot analysis of SARM in Daudi cell lysate with SARM antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | SARM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SARM Antibody: SARM, SAMD2, MyD88-5, KIAA0524, SARM, Sterile alpha and TIR motif-containing protein 1, Sterile alpha and Armadillo repeat protein |
| Product description | SARM Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. SARM (SAM and ARM-containing protein), along with other molecules such as TIRP, TRIF, TIRAP, and MyD88, is thought to serve as an adaptor protein for the TLRs that allows for the activation of downstream kinases and NF-κB, and ultimately the expression of proteins involved in host defense. While SARM has not been conclusively shown to associate directly with TLRs, the presence of a Toll-interluekin-1 (TIR) domain in SARM is consistent with a role as a signaling molecule.1) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. |
| Immunogen | SARM antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human SARM. The immunogen is located within amino acids 640 - 690 of SARM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SARM antibody can be used for detection of SARM by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SARM1 |
|---|---|
| Alternative Names | SARM Antibody: SARM SAMD2 MyD88-5 KIAA0524 SARM Sterile alpha and TIR motif-containing protein 1 Sterile alpha and Armadillo repeat protein |
| Molecular Weight(MW) | Predicted: 80 kDa Observed: 85 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (86%) |
Application
Immunocytochemistry staining of Daudi cells using SARM antibody at 2 μg/mL.
Application
Immunofluorescence of SARM in Daudi cells with SARM antibody at 2 μg/mL.
Application
Western blot analysis of SARM in Daudi cell lysate with SARM antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | SARM antibody can be used for detection of SARM by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SARM antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SARM Antibody is supplied in PBS containing 0.02% sodium azide. |
