WB
Various lysates were subjected to SDS PAGE followed by western blot with SARM1 antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using SARM1 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SARM1 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).FC
1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SARM1 Polyclonal antibody and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | SARM1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SARM1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:3000 IHC:1:200-1:800 ICC/IF:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Symbol | SARM1 |
|---|---|
| Gene Synonyms | SARM HsTIR SAMD2 hSARM1 MyD88-5 |
| Gene Full Name | sterile alpha and TIR motif containing 1 |
| Gene Summary | Enables NAD+ nucleosidase activity; NAD+ nucleosidase activity, cyclic ADP-ribose generating; and identical protein binding activity. Involved in NAD catabolic process and response to axon injury. Is active in mitochondrion. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | EC:3.2.2.- EC:3.2.2.6 FLJ36296 hSARM1 HsTIR |
| Molecular Weight(MW) | 70-80 kDa |
| Cellular Localization | Cell projection,Cytoplasm,Mitochondrion,Synapse. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SARM1 antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using SARM1 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SARM1 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).
FC
1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SARM1 Polyclonal antibody and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:500-1:3000 IHC:1:200-1:800 ICC/IF:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
