WB
Western blot analysis of SARM1 on different lysates with Rabbit anti-SARM1 antibody at 1/1,000 dilution. Exposure time: 25 seconds; Negative control: HaCaT, rat heart. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: SARM1 Recombinant Rabbit Monoclonal Antibody, 1/1,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Colon. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: SARM1 Recombinant Rabbit Monoclonal Antibody, 1/500, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cerebral cortex. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: SARM1 Recombinant Rabbit Monoclonal Antibody, 1/500, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: SH-SY5Y (Human neuroblastoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: SARM1 Recombinant Rabbit Monoclonal Antibody, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).| Product Name | SARM1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:500 ICC/IF:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SARM1 |
|---|---|
| Gene Synonyms | SARM HsTIR SAMD2 hSARM1 MyD88-5 |
| Gene Full Name | sterile alpha and TIR motif containing 1 |
| Gene Summary | Enables double-stranded DNA binding activity; hydrolase activity, hydrolyzing N-glycosyl compounds; and identical protein binding activity. Involved in NAD+ catabolic process; positive regulation of programmed cell death; and response to axon injury. Located in cytoplasm. Is active in mitochondrion. [provided by Alliance of Genome Resources, Jun 2026] |
| Alternative Names | FLJ36296 antibody KIAA0524 antibody MyD88-5 antibody SAM and ARM-containing protein antibody SAM domain-containing protein 2 antibody SAMD2 antibody SARM 1 antibody SARM1 antibody SARM1_HUMAN antibody Sterile alpha and Armadillo repeat protein antibody sterile alpha and HEAT/Armadillo motif protein ortholog of Drosophila antibody sterile alpha and HEAT/Armadillo motifs-containing protein antibody sterile alpha and TIR motif containing 1 antibody Sterile alpha and TIR motif-containing protein 1 antibody Sterile alpha and TIR motifs-containing protein 1 antibody Sterile alpha motif domain-containing protein 2 antibody Tir-1 homolog antibody |
| Molecular Weight(MW) | 79 kDa(Observed band size: ~79 kDa) |
| Cellular Localization | Cytoplasm, Cell projection, axon, dendrite, Synapse, Mitochondrion. |
| SwissProt ID | Q6SZW1 |
|---|
WB
Western blot analysis of SARM1 on different lysates with Rabbit anti-SARM1 antibody at 1/1,000 dilution. Exposure time: 25 seconds; Negative control: HaCaT, rat heart. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: SARM1 Recombinant Rabbit Monoclonal Antibody, 1/1,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Colon. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: SARM1 Recombinant Rabbit Monoclonal Antibody, 1/500, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cerebral cortex. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: SARM1 Recombinant Rabbit Monoclonal Antibody, 1/500, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: SH-SY5Y (Human neuroblastoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: SARM1 Recombinant Rabbit Monoclonal Antibody, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).| Application Notes | WB:1:1000 IHC:1:500 ICC/IF:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
