Application
Immunohistochemistry of SCO1 in human brain tissue with SCO1 antibody at 2.5 μg/mL.Application
Immunofluorescence of SCO1 in Human Brain cells with SCO1 antibody at 20 μg/mL.Application
Western blot analysis of SCO1 in human brain tissue lysate with SCO1 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | SCO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SCO1 Antibody: Synthesis of cytochrome c oxidase 1 was initially identified in yeast as one of two cytochrome c oxidase (COX) assembly proteins that enable the assembly of cytochrome c holoenzyme, a complex that catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. Like their yeast homologs, the function of both SCO1 and SCO2 are dependent on copper ion binding. Mutations in either gene can lead to cytochrome c oxidase respiratory chain defects, with a missense mutation in human SCO1 (P174L) associated with a fatal neonatal hepatopathy when the second allele is also non-functional, suggesting the pathology is due to loss of function. It has been suggested that this mutation alters the SCO1 affinity for the copper (I) ion, thus impairing the efficiency of copper transfer to the cytochrome c oxidase. At least two isoforms of SCO1 are known to exist and both are recognized by the SCO1 antibody. This SCO1 antibody has no cross-reactivity to SCO2.1) Glerum DM, Shtanko A, and Tzagoloff A. SCO1 and SCO2 act as high copy suppressors of a mitochondrial copper recruitment defect in Saccharomyces cerevisiae.J. Biol. Chem.1996; 271:20531-5. |
| Immunogen | SCO1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human SCO1. The immunogen is located within amino acids 100 - 150 of SCO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SCO1 antibody can be used for detection of SCO1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCO1 |
|---|---|
| Alternative Names | SCO1 Antibody: SCOD1 SCOD1 Protein SCO1 homolog mitochondrial |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of SCO1 in human brain tissue with SCO1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of SCO1 in Human Brain cells with SCO1 antibody at 20 μg/mL.
Application
Western blot analysis of SCO1 in human brain tissue lysate with SCO1 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | SCO1 antibody can be used for detection of SCO1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SCO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SCO1 Antibody is supplied in PBS containing 0.02% sodium azide. |
