Application
Immunohistochemistry of SCO2 in human liver tissue with SCO2 antibody at 2.5 μg/mL.Application
Western blot analysis of SCO2 in HL60 cell lysate lysate with SCO2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of SCO2 in Human Liver tissue with SCO2 antibody at 20 μg/mL.| Product Name | SCO2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SCO2 Antibody: Synthesis of cytochrome c oxidase 2 was initially identified in yeast as one of two cytochrome c oxidase (COX) assembly proteins that enable the assembly of cytochrome c holoenzyme, a complex that catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. Like their yeast homologs, the function of both SCO2 and SCO1 are dependent on copper ion binding. Recent studies suggest that SCO2 expression is regulated by p53, so that a decrease in p53 expression, such as in numerous tumors and cells lines, the drop in SCO2 expression leads to a shift from normal aerobic respiration towards the production of glycolytic ATP. Defects in the SCO2 protein are also associated with fatal infantile cardioencephalomyopathy and COX deficiency.1) Glerum DM, Shtanko A, and Tzagoloff A. SCO1 and SCO2 act as high copy suppressors of a mitochondrial copper recruitment defect in Saccharomyces cerevisiae. J. Biol. Chem. 1996; 271:20531-5. |
| Immunogen | SCO2 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human SCO2. The immunogen is located within the last 50 amino acids of SCO2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SCO2 antibody can be used for detection of SCO2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCO1 |
|---|---|
| Alternative Names | SCO2 Antibody: SCOD1 Protein SCO2 homolog mitochondrial |
| Molecular Weight(MW) | Predicted: 29 kDa Observed: 33 kDa |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Immunohistochemistry of SCO2 in human liver tissue with SCO2 antibody at 2.5 μg/mL.
Application
Western blot analysis of SCO2 in HL60 cell lysate lysate with SCO2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of SCO2 in Human Liver tissue with SCO2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1209 - HL60 Cell Lysate |
|---|---|
| Application Notes | SCO2 antibody can be used for detection of SCO2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SCO2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SCO2 Antibody is supplied in PBS containing 0.02% sodium azide. |
