Application
Immunohistochemistry of SEC62 in mouse brain tissue with SEC62 antibody at 5 μg/mL.Application
Immunofluorescence of SEC62 in mouse brain tissue with SEC62 antibody at 20 μg/mL.Application
Western blot analysis of SEC62 in rat brain tissue lysate with SEC62 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | SEC62 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SEC62 Antibody: SEC62 is an integral membrane protein located in the rough endoplasmic reticulum (ER) and is part of the SEC61-SEC62-SEC63 complex that is the central component of the protein translocation apparatus of the ER membrane. It is speculated that SEC61-SEC62-SEC63 may perform post-translational protein translocation into the ER and might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. Silencing of this gene with RNAi dramatically reduced the migration and invasive potential of numerous tumor cell lines, suggesting that it may be an attractive target for therapy of various tumors.1) Deshaies RJ and Scheckman R. SEC62 encodes a putative membrane protein required for protein translocation in the yeast endoplasmic reticulum. J. Cell Biol. 1989; 109:2653-64. |
| Immunogen | SEC62 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SEC62. The immunogen is located within amino acids 270 - 320 of SEC62. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SEC62 antibody can be used for detection of SEC62 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SEC62 |
|---|---|
| Alternative Names | SEC62 Antibody: HTP1 TP-1 Dtrp1 TLOC1 Translocation protein SEC62 Translocation protein 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (100%) |
| Tissue Specificity | SEC62 antibody is predicted to not cross-react with other SEC proteins. Multiple isoforms of SEC62 are known to exist. |
Application
Immunohistochemistry of SEC62 in mouse brain tissue with SEC62 antibody at 5 μg/mL.
Application
Immunofluorescence of SEC62 in mouse brain tissue with SEC62 antibody at 20 μg/mL.
Application
Western blot analysis of SEC62 in rat brain tissue lysate with SEC62 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | SEC62 antibody can be used for detection of SEC62 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SEC62 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SEC62 Antibody is supplied in PBS containing 0.02% sodium azide. |
