Application
Immunohistochemistry of SGK1 in human stomach tissue with SGK1 antibody at 5 μg/mL.Application
Immunofluorescence of SGK1 in human stomach tissue with SGK1 antibody at 20 μg/mL.Application
Western blot analysis of SGK1 in human stomach tissue lysate with SGK1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SGK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SGK1 Antibody: Serum and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases that plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. SGK1 functions as a regulator of cell survival and ion channels and transporters such as ENaC or KCNA3/Kv1.3. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.1) Webster MK, Goya L, Ge Y, et al. Characterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum. Mol. Cell Biol. 1993; 13:2031-40. |
| Immunogen | SGK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SGK1. The immunogen is located within amino acids 370 - 420 of SGK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SGK1 antibody can be used for detection of SGK1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SGK1 |
|---|---|
| Alternative Names | SGK1 Antibody: SGK SGK |
| Sequence Similarities | 2) Cat. No. 10-801 - Human Stomach Tissue Slide |
| Tissue Specificity | SGK1 antibody is predicted to not cross-react with other SGK protein family members. At least five isoforms are known to exist |
Application
Immunohistochemistry of SGK1 in human stomach tissue with SGK1 antibody at 5 μg/mL.
Application
Immunofluorescence of SGK1 in human stomach tissue with SGK1 antibody at 20 μg/mL.
Application
Western blot analysis of SGK1 in human stomach tissue lysate with SGK1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1319 - Human Stomach Tissue Lysate |
|---|---|
| Application Notes | SGK1 antibody can be used for detection of SGK1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SGK1 antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SGK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
