Application
Immunohistochemistry of SHISA9 in human brain tissue with SHISA9 antibody at 2.5 μg/mL.Application
Immunofluorescence of SHISA9 in human brain tissue with SHISA9 antibody at 20 μg/mL.Application
Western blot analysis of SHISA9 in rat brain tissue lysate with SHISA9 antibody at 1 μg/mL.| Product Name | SHISA9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SHISA9 Antibody: Shisa proteins are a recently-identified family of modulators of both FGF and Wnt signaling that block both maturation and transport to the cell surface of their respective receptors. SHISA9, also known as CKAMP44a, is a brain-specific type I transmembrane protein that associates with AMPA receptors in synaptic spines and promotes AMPA receptor desensitization at excitatory synapses. It is thought to modulate short-term plasticity at specific excitatory synapses.1) Hedge TA and Mason I. Expression of Shisa2, a modulator of both Wnt and Fgf signaling, in the chick embryo. Int. J. Dev. Biol.2008; 52:81-5. |
| Immunogen | SHISA9 antibody was raised against a 22 amino acid synthetic peptide near the center of human SHISA9. The immunogen is located within amino acids 120 - 170 of SHISA9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SHISA9 antibody can be used for detection of SHISA9 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHISA9 |
|---|---|
| Alternative Names | SHISA9 Antibody: CKAMP44 Protein shisa-9 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Entrez Gene | 729993 |
|---|---|
| Protein Accession | NP_001138676 |
Application
Immunohistochemistry of SHISA9 in human brain tissue with SHISA9 antibody at 2.5 μg/mL.
Application
Immunofluorescence of SHISA9 in human brain tissue with SHISA9 antibody at 20 μg/mL.
Application
Western blot analysis of SHISA9 in rat brain tissue lysate with SHISA9 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | SHISA9 antibody can be used for detection of SHISA9 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SHISA9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SHISA9 Antibody is supplied in PBS containing 0.02% sodium azide. |
