ICC/IF
Immunofluorescence staining of MCF-7 cells with SHISA9 Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-SHISA9 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Protein shisa-9 protein (171-424AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHISA9 |
|---|---|
| Gene Synonyms | CKAMP44 |
| Gene Full Name | shisa family member 9 |
| Gene Summary | Predicted to enable PDZ domain binding activity. Predicted to be involved in regulation of postsynaptic neurotransmitter receptor activity and regulation of short-term neuronal synaptic plasticity. Predicted to act upstream of or within regulation of AMPA receptor activity. Predicted to be located in synapse. Predicted to be part of AMPA glutamate receptor complex. Predicted to be active in dendritic spine membrane; glutamatergic synapse; and postsynaptic density membrane. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | 2700045P11Rik antibody CKAMP44 antibody Cystine knot AMPAR modulating protein antibody Cystine knot AMPAR receptor-modulating protein 44-KD antibody DKFZp686D24206 antibody DKFZp686E24206 antibody FLJ10015 antibody FLJ37653 antibody FLJ58277 antibody FLJ99079 antibody FLJ99234 antibody Protein shisa-9 antibody shisa family member 9 antibody Shisa homolog 9 (Xenopus laevis) antibody Shisa homolog 9 antibody shisa Xenopus homolog of 9 antibody shisa9 antibody SHSA9_HUMAN antibody |
| Cellular Localization | Cell projection, dendritic spine membrane; Single-pass type I membrane protein. Cell junction, synapse. |
ICC/IF
Immunofluorescence staining of MCF-7 cells with SHISA9 Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
