Application
SHMT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in 293 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | SHMT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.1) Porter, K.E., et al. Environ. Res. 110(6):580-587(2010) |
| Immunogen | This SHMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-47 amino acids from the N-terminal region of human SHMT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHMT1 |
|---|---|
| Alternative Names | Serine hydroxymethyltransferase cytosolic SHMT Glycine hydroxymethyltransferase Serine methylase SHMT1 |
| Molecular Weight(MW) | 53 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
SHMT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in 293 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
