WB
Western blot, All lanes: SHMT1 antibody at 7µg/ml, Lane 1: Hela whole cell lysate, Lane 2: HepG2 whole cell lysate, Secondary, Goat polyclonal to rabbit IgG at 1/10000 dilution.IHC
Immunohistochemistry of paraffin-embedded human colon tissue using SHMT1 Antibody at dilution of 1:100.| Product Name | SHMT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Serine hydroxymethyltransferase, cytosolic protein (324-483AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:20-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHMT1 |
|---|---|
| Gene Synonyms | SHMT CSHMT hcSHMT |
| Gene Full Name | serine hydroxymethyltransferase 1 |
| Gene Summary | This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| Molecular Weight(MW) | 54/50/45/38kDa(Observed MW 54kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot, All lanes: SHMT1 antibody at 7µg/ml, Lane 1: Hela whole cell lysate, Lane 2: HepG2 whole cell lysate, Secondary, Goat polyclonal to rabbit IgG at 1/10000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human colon tissue using SHMT1 Antibody at dilution of 1:100.| Application Notes | WB:1:500-1:2000 IHC:1:20-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
